Publications

* Full open access.
# Free from PMC.

Selected publications – last 3 years
DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes. *
Glover L, Alsford S and Horn D (2013) PLoS Pathogens 9:e1003260
Aquaglyceroporin 2 controls susceptibility to melarsoprol and pentamidine in African trypanosomes. *
Baker N, Glover L, Munday JC, Aguinaga Andrés D, Barrett MP, de Koning HP, and Horn D (2012) Proc. Natl. Acad. Sci. USA 109:10996–11001
High-throughput decoding of antitrypanosomal drug efficacy and resistance. #
Alsford S, Eckert S, Baker N, Glover L, Sanchez-Flores A, Leung K, Turner DJ, Field MC, Berriman M & Horn D. (2012) Nature 482:232-236 PMC
High throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. *
Alsford S, Turner DJ., Obado SO, Sanchez-Flores A, Glover L, Berriman M, Hertz-Fowler C & Horn D. (2011) Genome Research 21:915-924
All publications
2014
Review: Antiparasitic Chemotherapy: From Genomes to Mechanisms.
Horn D. & Duraisingh MT. (2013) Annual Review of Pharmacology and Toxicology In Press / Pubmed:
2013
PPL2 translesion polymerase is essential for the completion of chromosomal DNA replication in the African trypanosome. *
Rudd SG, Glover L, Jozwiakowski SK, Horn D and Doherty AJ (2013) Mol. Cell In press:
Trypanosoma brucei Aquaglyceroporin 2 is a high affinity transporter for pentamidine and melaminophenyl arsenic drugs and the main genetic determinant of resistance to these drugs. *
Munday J, Eze A, Baker N, Glover L, Clucas C, Aguinaga Andres D, Natto M, Teka I, McDonald J, Lee R, Graf F, Ludin P, Burchmore R, Turner MC, Tait A, McLeod A, Mäser P, Barrett M, Horn D, de Koning HP (2013) J. of Antimicrob. Chemother. In press: Pubmed
Aquaporin 2 mutations in Trypanosoma brucei gambiense field isolates correlate with decreased susceptibility to pentamidine and melarsoprol. *
Graf FE, Ludin P, Wenzler T, Kaiser M, Brun R, Pyana PP, Büscher P, de Koning HP, Horn D, Mäser P. (2013) PLoS Neglected Tropical Diseases 7:e2475
Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNAi library screening for resistance to the phosphodiesterase inhibitor Cpd A. *
Gould MK, Bachmaier S, Ali JA, Alsford S, Tagoe DN, Munday JC, Schnaufer AC, Horn D, Boshart M, de Koning HP (2013) Antimicrob. Agents Chemother. 57:4882-93
DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes. *
Glover L, Alsford S and Horn D (2013) PLoS Pathogens 9:e1003260
Review: Antigenic variation in African trypanosomes: the importance of chromosomal and nuclear context in VSG expression control. *
Glover L, Hutchinson S, Alsford S, McCulloch R, Field MC & Horn D. (2013) Cell. Microbiol. 15:1984-93:
Review [BSP meeting]: High-throughput decoding of drug targets and drug resistance mechanisms in African trypanosomes.
Horn D. (2013) Parasitology In Press / Pubmed:
Review: Genetic dissection of drug resistance in trypanosomes. *
Alsford S, Kelly JM, Baker N, & Horn D. (2013) Parasitology 140:1478-1491
Opinion: Receptor mediated endocytosis for drug delivery in African Trypanosomes: fulfilling Paul Ehrlich’s vision of chemotherapy.
Alsford S, Field MC, & Horn D. (2013) Trends in Parasitology 29:207-212
Review: Drug resistance in African trypanosomiasis: the melarsoprol and pentamidine story. #
Baker N, de Koning HP, Mäser P, & Horn D. (2013) Trends in Parasitol. 29:110-118
2012
Cell cycle regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei. *
Alsford S and Horn D (2012) Nucleic Acids Res. 40:10150-60
Aquaglyceroporin 2 controls susceptibility to melarsoprol and pentamidine in African trypanosomes. *
Baker N, Glover L, Munday JC, Aguinaga Andrés D, Barrett MP, de Koning HP, and Horn D (2012) Proc. Natl. Acad. Sci. USA 109:10996–11001
Identification of a coiled-coil lamin-like protein in Trypanosoma brucei. *
duBois K, Alsford S, Buisson J, Swiderski M, Bart J, Bastin P, Barry D, Navarro M, Horn D, Aitchison J, Rout M and Field M. (2012) PLOS Biology 10:e1001287
Histone deacetylase inhibitors with potent activity against the African trypanosome Trypanosoma brucei. *
Kelly J, Taylor M, Horn D, Loza E, Kalvinsh I and Björkling F. (2012) Bioorganic & Medicinal Chemistry Letters 22:1886-90
Trypanosomal histone gamma-H2A and the DNA damage response. *
Glover L and Horn D. (2012) Mol. Biochem. Parasitol. 183:78-83
High-throughput decoding of antitrypanosomal drug efficacy and resistance. #
Alsford S, Eckert S, Baker N, Glover L, Sanchez-Flores A, Leung K, Turner DJ, Field MC, Berriman M & Horn D. (2012) Nature 482:232-236 PMC
Extraview: Telomeres, tethers and trypanosomes. *
Field MC, Horn D, Alsford S, Koreny L & Rout MP. (2012) Nucleus 3:1-9
Review: Epigenetic mechanisms, nuclear architecture and the control of gene expression in trypanosomes.
Alsford S, Dubois K, Horn D & Field MC. (2012) Expert Rev Mol Med 14:e13
2011
High throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. *
Alsford S, Turner DJ., Obado SO, Sanchez-Flores A, Glover L, Berriman M, Hertz-Fowler C & Horn D. (2011) Genome Research 21:915-924
Elongator protein 3b negatively regulates ribosomal DNA transcription in African trypanosomes. *
Alsford S & Horn D. (2011) Mol Cell Biol 31:1822-1832
Microhomology mediated deletion and gene conversion in African trypanosomes. *
Glover L, Jun J & Horn D. (2011) Nucleic Acids Res 39:1372-1380
Genome-wide RNAi screens in African trypanosomes identify the nifurtimox activator NTR and the eflornithine transporter AAT6. *
Baker N, Alsford S & Horn D. (2011) Mol Biochem Parasitol 176:55-57
2010
Histone deacetylases play distinct roles in telomeric VSG expression site silencing in African trypanosomes. *
Wang Q-P, Kawahara T and Horn D. (2010) Mol Microbiol 77:1237-1245
RNA interference, growth and differentiation appear normal in African trypanosomes lacking Tudor staphylococcal nuclease. *
Alsford S, Kemp LE, Kawahara T and Horn D. (2010) Mol Biochem Parasitol 174:70-73
Review: Molecular mechanisms underlying the control of antigenic variation in African trypanosomes. *
Horn D and McCulloch R. (2010) Curr Opin Microbiol 13:700-705
2009
Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei. *
Glover L and Horn D. (2009) Mol Biochem Parasitol 166:194-197
Opinion: What has DNA sequencing revealed about the VSG Expression Sites of African Trypanosomes?
McCulloch R and Horn D. (2009) Trends Parasitol 8:359-363
Dispatch: Antigenic variation: Extending the reach of telomeric silencing. *
Horn D. (2009) Curr Biol 19:R496-498
Minireview: DNA breaks as triggers for antigenic variation in African trypanosomes. *
Alsford S, Horn D and Glover L. (2009) Genome Biol 10:223
2008
Two essential MYST-family proteins display distinct roles in histone H4K10 acetylation and telomeric silencing in trypanosomes. *
Kawahara T, Siegel TN, Ingram AK, Alsford S, Cross GAM and Horn D. (2008) Mol Microbiol 69:1054-1068
Single-locus targeting vectors for regulated transgene expression and RNAi in T. brucei. #
Alsford S and Horn D. (2008) Mol Biochem Parasitol 161:76-79
A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes. *
Wilkinson SR, Taylor MC, Horn D, Kelly JM and Cheeseman I. (2008) Proc Natl Acad Sci USA 105:5022-5027
Sequence homology and microhomology dominate chromosomal double-strand break repair in African trypanosomes. *
Glover L, McCulloch R and Horn D. (2008) Nucleic Acids Res 36:2608-2618
Acetylation of histone H4K4 is cell-cycle regulated and mediated by HAT3 in Trypanosoma brucei. *
Siegel TN, Kawahara T, DeGrasse JA, Janzen CJ, Horn D and Cross GAM. (2008) Mol Microbiol 67:762-771
Codon usage suggests that translational selection has a major impact on protein expression in trypanosomatids. *
Horn D. (2008) BMC Genomics 9:2
Book Chapter: Analysis of small GTPase function in trypanosomes.
Field MC, Horn D and Carrington M (2008) Elsevier. Methods Enzymol 438:57-76
Book Chapter: Histone Deacetylases.
Horn D (2008) Landes Bioscience. Drug targets in kinetoplastid parasites Chapter.
2007
RNA polymerase I transcription stimulates homologous recombination in Trypanosoma brucei. #
Alsford S and Horn D. (2007) Mol Biochem Parasitol 153:77-9
Deletion of a trypanosome telomere leads to loss of silencing and progressive loss of terminal DNA in the absence of cell cycle arrest. *
Glover L, Alsford S, Beattie C and Horn D. (2007) Nucleic Acids Res 35:872-80
A sirtuin in the African trypanosome is involved in both DNA repair and telomeric gene silencing but is not required for antigenic variation. *
Alsford S, Kawahara T, Isamah C and Horn D. (2007) Mol Microbiol 63:724-36
Deletion of the Trypanosoma brucei superoxide dismutase gene Tbsodb1 increases sensitivity to nifurtimox and benznidazole. *
Prathalingham SR, Wilkinson SR, Horn D and Kelly JM. (2007) Antimicrob Agents Chemother 51:755-58
Research Focus: Introducing histone modification in trypanosomes. #
Horn D. (2007) Trends Parasitol 23:239-42
Book Chapter: Chromosome structure and dynamics.
Renauld H, Kelly JM and Horn D (2007) Horizon Press. Trypanosomes – after the genome Chapter 4.
2006
Chromsome-wide analysis of gene function by RNA interference in the African trypanosome. *
Subramaniam C, Veazey P, Redmond S, Hayes-Sinclair J, Chambers E, Carrington M, Gull K, Matthews K, Horn D and Field MC. (2006) Eukaryotic Cell 5:1539-1549
Functional characterisation of the iron superoxide dismutase gene repertoire in Trypanosoma brucei.
Wilkinson SR, Prathalingam SR, Taylor MC, Ahmed A, Horn D, Kelly JM (2006) Free Radic Biol Med 40:198-209
Repression of polymerase I-mediated gene expression at Trypanosoma brucei telomeres. *
Glover L, Horn D (2006) EMBO Rep 7:93-9
2005
Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects. #
Alsford S, Kawahara T, Glover L, Horn D (2005) Mol Biochem Parasitol 144:142-8
Vitamin C biosynthesis in trypanosomes: a role for the glycosome. *
Wilkinson SR, Prathalingam SR, Taylor MC, Horn D, Kelly JM (2005) Proc Natl Acad Sci USA 102:11645-50
The genome of the kinetoplastid parasite, Leishmania major. #
Ivens AC … Horn D … Myler PJ (2005) Science 309:436-42
The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM … Horn D … Andersson B (2005) Science 309:409-15
Directional enrichment of directly cloned PCR products. *
Horn D (2005) Biotechniques 39:40-46.
Multiplex analysis of RNA interference defects in Trypanosoma brucei. #
Alsford S, Glover L, Horn D (2005) Mol Biochem Parasitol 139:129-32
A doubly inducible system for RNA interference and rapid RNAi plasmid construction in Trypanosoma brucei.
Alibu VP, Storm L, Haile S, Clayton C, Horn D (2005) Mol Biochem Parasitol 139:75-82
Review: The central roles of telomeres and subtelomeres in antigenic variation in African trypanosomes. *
Horn D, Barry JD (2005) Chromosome Res 13:525-33
Book Chapter: Down-regulating gene expression by RNA interference in Trypanosoma brucei.
Clayton CE, Estevez AM, Hartmann C, Alibu VP, Field M, Horn D (2005) Methods Mol Biol 309:39-60
pre-2005
Review: The molecular control of antigenic variation in Trypanosoma brucei.
Horn D (2004) Curr Mol Med 4:563-76
Review: Trypanosomatid Histones. *
Alsford S, Horn D (2004) Mol Micro 53:365-72
RNAi identifies two hydroperoxide metabolising enzymes that are essential to the bloodstream form of the African trypanosome. *
Wilkinson SR, Horn D, Prathalingam R, and Kelly JM (2003) J Biol Chem 278:31640-6
Histone deacetylases in Trypanosoma brucei: Two are essential and another is required for normal cell cycle progression. *
Ingram AK and Horn D (2002) Mol Microbiol 45:89-97
Review: Nuclear gene transcription and chromatin in Trypanosoma brucei
Horn D (2001) Int J Parasitol 31:1157-65
Telomere maintenance and length regulation in Trypanosoma brucei. *
Horn D, Spence C and Ingram AK (2000) EMBO J 19:2332-9
Genetic manipulation indicates that ARD1 is an essential N-terminal protein acetyltransferase in Trypanosoma brucei.
Ingram AK, Cross GAM and Horn D (2000) Mol Biochem Parasitol 111:309-17
Position-dependent and promoter-specific regulation of gene expression in Trypanosoma brucei. *
Horn D, Cross GAM (1997) EMBO J 16:7422-31
Analysis of Trypanosoma brucei VSG expression site switching in vitro.
Horn D, Cross GAM (1997) Mol Biochem Parasitol 84:189-201
A developmentally regulated position effect at a telomeric locus in Trypanosoma brucei. *
Horn D, Cross GAM (1995) Cell 83:555-61
Mice lacking Snrpn expression show normal regulation of neuronal alternative splicing events.
Huntriss JD, Barr JA, Horn D, Williams DG, Latchman DS (1994) Mol. Biol. Rep. 20:19-25
The cardiac form of the tissue-specific protein SmN is identical to the brain and embryonic forms of the protein.
Gerrelli D, Grimaldi K, Horn D, Mahadeva U, Sharpe N, Latchman DS (1993) J. Mol. Cell. Cardiol. 25:321-329
Expression of the SmN splicing protein is developmentally regulated in the rodent brain but not in the rodent heart.
Grimaldi K, Horn D, Hudson LD, Terenghi G, Polak JM, Latchman DS (1993) Dev. Biol. 156:319-323
The tissue specific SmN protein does not influence the alternative splicing of endogenous N-Cam and C-SRC RNAs in transfected 3T3 cells.
Horn D, Latchman DS (1993) Brain Res: Mol. Brain Res. 19:181-187
Alternative splicing of the mRNA encoding the a subunits of the G(o) GTP-binding protein during brain development and in neuronal cell lines.
Horn D, Latchman DS (1993) Neurosci. Lett. 155:57-60
A distal region of the CALC-I gene is necessary for regulated alternative splicing.
Horn D, Latchman DS (1993) FEBS Lett. 324:123-126
Redistribution of secretory granule components precedes that of synaptic vesicle proteins during differentiation of a neuronal cell line in serum-free medium.
Wheatley SC, Suburo AM, Horn D, Vucicevic V, Terenghi G, Polak JM, Latchman DS. (1992) Neuroscience. 51:575-582
Expression of the tissue specific splicing protein SmN in neuronal cell lines and in regions of the brain with different splicing capacities.
Horn D, Suburo A, Terenghi G, Hudson L, Polak JM, Latchman DS. (1992) Brain Res: Mol. Brain Res. 19:13-19
Intracellular redistribution of neuropeptides and secretory proteins during differentiation of neuronal cell lines.
Suburo AM, Wheatley SC, Horn D, Gibson SJ, Jahn R, Fischer-Colbrie R, Wood JN, Latchman DS, Polak JM. (1992) Neuroscience. 46:881-889
Neuronal expression of the tissue specific splicing protein SmN and its possible role in pre mRNA splicing.
Horn D, Latchman DS. (1992) Biochem. Soc. Trans. 20:266s