{"id":123,"date":"2012-10-12T13:35:15","date_gmt":"2012-10-12T12:35:15","guid":{"rendered":"http:\/\/blogs.lshtm.ac.uk\/alsfordlab\/?page_id=123"},"modified":"2025-11-24T09:51:16","modified_gmt":"2025-11-24T09:51:16","slug":"human-serum-resistance","status":"publish","type":"page","link":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/research\/human-serum-resistance\/","title":{"rendered":"Trypanosomes &amp; TLF"},"content":{"rendered":"<p>We are particularly interested in the transport of the trypanolytic factors (TLFs) from the flagellar pocket to&nbsp; the lysosome (their point of action), and the proteins and pathways involved in this process. Work by others has shown that TLF1 enters the cell via the TbHpHb receptor, and that ApoL1 (a component of TLF1\/2) causes cell lysis by forming pores in the lysosomal membrane, while the expression of the serum resistance antigen&nbsp;renders <em>T. b. rhodesisense<\/em> resistant to human serum. However, there are significant gaps in our knowledge about this process and the means by which African trypanosomes can become resistant to lysis by human serum. For example, how does TLF2 enter the cell? What components of the cell\u2019s endocytic machinery are responsible for the intracellular transit of TLF to the lysosome? How is the lytic component (ApoL1) released from the TLF particle? What is the molecular basis for <em>T. b. gambiense<\/em> resistance to TLF\/ApoL1? Though changes in the TbHpHb receptor seem to be involved, this isn&#8217;t the whole story.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>We are particularly interested in the transport of the trypanolytic factors (TLFs) from the flagellar&#8230;<\/p>\n","protected":false},"author":116,"featured_media":0,"parent":31,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-123","page","type-page","status-publish","hentry","odd"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.9 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Trypanosomes &amp; TLF - Alsford Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/research\/human-serum-resistance\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Trypanosomes &amp; TLF - Alsford Lab\" \/>\n<meta property=\"og:description\" content=\"We are particularly interested in the transport of the trypanolytic factors (TLFs) from the flagellar...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/research\/human-serum-resistance\/\" \/>\n<meta property=\"og:site_name\" content=\"Alsford Lab\" \/>\n<meta property=\"article:modified_time\" content=\"2025-11-24T09:51:16+00:00\" \/>\n<meta name=\"twitter:label1\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/research\/human-serum-resistance\/\",\"url\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/research\/human-serum-resistance\/\",\"name\":\"Trypanosomes &amp; TLF - Alsford Lab\",\"isPartOf\":{\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/#website\"},\"datePublished\":\"2012-10-12T12:35:15+00:00\",\"dateModified\":\"2025-11-24T09:51:16+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/research\/human-serum-resistance\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/research\/human-serum-resistance\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/research\/human-serum-resistance\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Research\",\"item\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/research\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Trypanosomes &amp; 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