{"id":163,"date":"2012-10-16T12:04:38","date_gmt":"2012-10-16T11:04:38","guid":{"rendered":"http:\/\/blogs.lshtm.ac.uk\/alsfordlab\/?page_id=163"},"modified":"2025-11-24T09:55:11","modified_gmt":"2025-11-24T09:55:11","slug":"tom-x","status":"publish","type":"page","link":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/","title":{"rendered":"TOM-X"},"content":{"rendered":"<p><span style=\"font-family: arial\">Cassette sequenced in full<em><\/em><\/span><\/p>\n<p><span style=\"font-family: arial\"><em>Hin<\/em>dIII &#8212; START <em>tdTomato<\/em> &#8212; <em>Xba<\/em>I &#8212; 1998 bp stuffer &#8212; <em>Bam<\/em>HI<\/span><\/p>\n<pre class=\"wp-block-preformatted\">    1  AAGCTTATGG TGAGCAAGGG CGAGGAGGTC ATCAAAGAGT TCATGCGCTT\n   51  CAAGGTGCGC ATGGAGGGCT CCATGAACGG CCACGAGTTC GAGATCGAGG\n  101  GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG\n  151  GTGACCAAGG GCGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCCCA\n  201  GTTCATGTAC GGCTCCAAGG CGTACGTGAA GCACCCCGCC GACATCCCCG\n  251  ATTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG\n  301  AACTTCGAGG ACGGCGGTCT GGTGACCGTG ACCCAGGACT CCTCCCTGCA\n  351  GGACGGCACG CTGATCTACA AGGTGAAGAT GCGCGGCACC AACTTCCCCC\n  401  CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCACC\n  451  GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG AAGGGCGAGA TCCACCAGGC\n  501  CCTGAAGCTG AAGGACGGCG GCCACTACCT GGTGGAGTTC AAGACCATCT\n  551  ACATGGCCAA GAAGCCCGTG CAACTGCCCG GCTACTACTA CGTGGACACC\n  601  AAGCTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA\n  651  CGAGCGCTCC GAGGGCCGCC ACCACCTGTT CCTGGGGCAT GGCACCGGCA\n  701  GCACCGGCAG CGGCAGCTCC GGCACCGCCT CCTCCGAGGA CAACAACATG\n  751  GCCGTCATCA AAGAGTTCAT GCGCTTCAAG GTGCGCATGG AGGGCTCCAT\n  801  GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG\n  851  AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC\n  901  TTCGCCTGGG ACATCCTGTC CCCCCAGTTC ATGTACGGCT CCAAGGCGTA\n  951  CGTGAAGCAC CCCGCCGACA TCCCCGATTA CAAGAAGCTG TCCTTCCCCG\n 1001  AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGTCTGGTG\n 1051  ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCACGCTGA TCTACAAGGT\n 1101  GAAGATGCGC GGCACCAACT TCCCCCCCGA CGGCCCCGTA ATGCAGAAGA\n 1151  AGACCATGGG CTGGGAGGCC TCCACCGAGC GCCTGTACCC CCGCGACGGC\n 1201  GTGCTGAAGG GCGAGATCCA CCAGGCCCTG AAGCTGAAGG ACGGCGGCCA\n 1251  CTACCTGGTG GAGTTCAAGA CCATCTACAT GGCCAAGAAG CCCGTGCAAC\n 1301  TGCCCGGCTA CTACTACGTG GACACCAAGC TGGACATCAC CTCCCACAAC\n 1351  GAGGACTACA CCATCGTGGA ACAGTACGAG CGCTCCGAGG GCCGCCACCA\n 1401  CCTGTTCCTG TACGGCATGG ACGAGCTGTA CAAGTCTAGA NNNNNNNNNN\n 1451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1601  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1651  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1701  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1801  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1851  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1901  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1951  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2001  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2051  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2601  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2651  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2701  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2801  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2851  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2901  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2951  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 3001  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 3051  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 3101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 3151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 3201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 3251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 3301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 3351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 3401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG ATCC<\/pre>\n","protected":false},"excerpt":{"rendered":"<p>Cassette sequenced in full HindIII &#8212; START tdTomato &#8212; XbaI &#8212; 1998 bp stuffer &#8212;&#8230;<\/p>\n","protected":false},"author":116,"featured_media":0,"parent":142,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-163","page","type-page","status-publish","hentry","odd"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.9 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>TOM-X - Alsford Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"TOM-X - Alsford Lab\" \/>\n<meta property=\"og:description\" content=\"Cassette sequenced in full HindIII &#8212; START tdTomato &#8212; XbaI &#8212; 1998 bp stuffer &#8212;...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/\" \/>\n<meta property=\"og:site_name\" content=\"Alsford Lab\" \/>\n<meta property=\"article:modified_time\" content=\"2025-11-24T09:55:11+00:00\" \/>\n<meta name=\"twitter:label1\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/\",\"url\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/\",\"name\":\"TOM-X - Alsford Lab\",\"isPartOf\":{\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/#website\"},\"datePublished\":\"2012-10-16T11:04:38+00:00\",\"dateModified\":\"2025-11-24T09:55:11+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Cell Lines &amp; Plasmids\",\"item\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"pRP series\",\"item\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"TOM-X\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/#website\",\"url\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/\",\"name\":\"Alsford Lab\",\"description\":\"Trypanosome molecular and cellular biology\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-GB\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"TOM-X - Alsford Lab","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/","og_locale":"en_GB","og_type":"article","og_title":"TOM-X - Alsford Lab","og_description":"Cassette sequenced in full HindIII &#8212; START tdTomato &#8212; XbaI &#8212; 1998 bp stuffer &#8212;...","og_url":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/","og_site_name":"Alsford Lab","article_modified_time":"2025-11-24T09:55:11+00:00","twitter_misc":{"Estimated reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/","url":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/","name":"TOM-X - Alsford Lab","isPartOf":{"@id":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/#website"},"datePublished":"2012-10-16T11:04:38+00:00","dateModified":"2025-11-24T09:55:11+00:00","breadcrumb":{"@id":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/tom-x\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Cell Lines &amp; Plasmids","item":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/"},{"@type":"ListItem","position":2,"name":"pRP series","item":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/"},{"@type":"ListItem","position":3,"name":"TOM-X"}]},{"@type":"WebSite","@id":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/#website","url":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/","name":"Alsford Lab","description":"Trypanosome molecular and cellular biology","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-GB"}]}},"_links":{"self":[{"href":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/wp-json\/wp\/v2\/pages\/163","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/wp-json\/wp\/v2\/users\/116"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/wp-json\/wp\/v2\/comments?post=163"}],"version-history":[{"count":4,"href":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/wp-json\/wp\/v2\/pages\/163\/revisions"}],"predecessor-version":[{"id":1783,"href":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/wp-json\/wp\/v2\/pages\/163\/revisions\/1783"}],"up":[{"embeddable":true,"href":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/wp-json\/wp\/v2\/pages\/142"}],"wp:attachment":[{"href":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/wp-json\/wp\/v2\/media?parent=163"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}