{"id":168,"date":"2012-10-16T12:12:14","date_gmt":"2012-10-16T11:12:14","guid":{"rendered":"http:\/\/blogs.lshtm.ac.uk\/alsfordlab\/?page_id=168"},"modified":"2025-11-24T09:55:47","modified_gmt":"2025-11-24T09:55:47","slug":"x-gfp","status":"publish","type":"page","link":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/x-gfp\/","title":{"rendered":"X-GFP"},"content":{"rendered":"<p><span style=\"font-family: arial\">Cassette sequenced in full<\/span><\/p>\n<p><strong>x<sup>GFP<\/sup> in pRP<\/strong><\/p>\n<p><em>Hin<\/em>dIII &#8212; 732 bp stuffer &#8212; <em>Xba<\/em>I &#8212; <em>eGFP<\/em> STOP &#8212; <em>Bam<\/em>HI<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: arial\"><strong>x<sup>GFP<\/sup> in pRPa<\/strong><\/span><\/p>\n<p><span style=\"font-family: arial\"><em>Pac<\/em>I <em>Hin<\/em>dIII &#8212; 389 bp stuffer &#8212; <em>Xba<\/em>I &#8212; <em>eGFP<\/em> STOP &#8212; <em>Bam<\/em>HI<\/span><\/p>\n<pre class=\"wp-block-preformatted\">    1  TTAATTAAGC TTNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n   51  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  401  NTCTAGAGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC\n  451  TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC\n  501  GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG\n  551  CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA\n  601  CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC\n  651  GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT\n  701  CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG\n  751  AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG\n  801  GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA\n  851  CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT\n  901  TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC\n  951  TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA\n 1001  CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC\n 1051  GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC\n 1101  GGCATGGACG AGCTGTACAA GTAAGGATCC<\/pre>\n","protected":false},"excerpt":{"rendered":"<p>Cassette sequenced in full xGFP in pRP HindIII &#8212; 732 bp stuffer &#8212; XbaI &#8212;&#8230;<\/p>\n","protected":false},"author":116,"featured_media":0,"parent":142,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-168","page","type-page","status-publish","hentry","odd"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.9 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>X-GFP - Alsford Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/x-gfp\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"X-GFP - Alsford Lab\" \/>\n<meta property=\"og:description\" content=\"Cassette sequenced in full xGFP in pRP HindIII &#8212; 732 bp stuffer &#8212; XbaI &#8212;...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/x-gfp\/\" \/>\n<meta property=\"og:site_name\" content=\"Alsford Lab\" \/>\n<meta property=\"article:modified_time\" content=\"2025-11-24T09:55:47+00:00\" \/>\n<meta name=\"twitter:label1\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/x-gfp\/\",\"url\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/x-gfp\/\",\"name\":\"X-GFP - Alsford Lab\",\"isPartOf\":{\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/#website\"},\"datePublished\":\"2012-10-16T11:12:14+00:00\",\"dateModified\":\"2025-11-24T09:55:47+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/x-gfp\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/x-gfp\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/prp-series\/x-gfp\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Cell Lines &amp; 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