{"id":220,"date":"2012-10-16T15:10:54","date_gmt":"2012-10-16T14:10:54","guid":{"rendered":"http:\/\/blogs.lshtm.ac.uk\/alsfordlab\/?page_id=220"},"modified":"2025-11-24T09:53:17","modified_gmt":"2025-11-24T09:53:17","slug":"blasticidin-s-deaminase2","status":"publish","type":"page","link":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/pnat-x-tag\/blasticidin-s-deaminase2\/","title":{"rendered":"Blasticidin-S Deaminase"},"content":{"rendered":"<p><span style=\"font-family: arial\">Cassette sequenced in full<\/span><\/p>\n<p><span style=\"font-family: arial\"><em>Kpn<\/em>I &#8212; START &#8212; <em>BSD<\/em> &#8212; STOP &#8212; <em>Eco<\/em>RI <em>Nhe<\/em>I<\/span><\/p>\n<pre class=\"wp-block-preformatted\">   1  GGTACCATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG\n  51  AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG\n 101  CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA\n 151  TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC\n 201  TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG\n 251  AGAACAGGGG CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC\n 301  GATCTGCATC CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC\n 351  GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG\n 401  GCTAAGAATT CGCTAGCGAT C<\/pre>\n","protected":false},"excerpt":{"rendered":"<p>Cassette sequenced in full KpnI &#8212; START &#8212; BSD &#8212; STOP &#8212; EcoRI NheI 1&#8230;<\/p>\n","protected":false},"author":116,"featured_media":0,"parent":211,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-220","page","type-page","status-publish","hentry","odd"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.9 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Blasticidin-S Deaminase - Alsford Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/pnat-x-tag\/blasticidin-s-deaminase2\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Blasticidin-S Deaminase - Alsford Lab\" \/>\n<meta property=\"og:description\" content=\"Cassette sequenced in full KpnI &#8212; 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