{"id":226,"date":"2012-10-16T15:17:53","date_gmt":"2012-10-16T14:17:53","guid":{"rendered":"http:\/\/blogs.lshtm.ac.uk\/alsfordlab\/?page_id=226"},"modified":"2025-11-24T09:52:08","modified_gmt":"2025-11-24T09:52:08","slug":"pnat-tag-x","status":"publish","type":"page","link":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/pnat-tag-x\/","title":{"rendered":"pNAT tag-X"},"content":{"rendered":"

Derived from known sequence<\/span><\/p>\n

\n\n\n\n\n\n\n\n\n\n\n
Landmarks<\/strong><\/span><\/td>\nAvailable expression cassettes<\/strong><\/span><\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
0001-2497: (2497)<\/span><\/td>\nVector backbone<\/span><\/td>\nSequence page<\/strong><\/span><\/td>\nExpression cassette<\/strong><\/span><\/td>\nRegion replaced<\/strong><\/span><\/td>\n<\/tr>\n
2498-2736: (239)<\/span><\/td>\nT. brucei<\/em> \u03b2\u03b1 tubulin mRNA processing 5′<\/span><\/td>\nN-terminal eGFP<\/a><\/span><\/td>\nGFP<\/sup>x<\/span><\/td>\nHin<\/em>dIII – Bam<\/em>HI<\/span><\/td>\n<\/tr>\n
2747-3145: (399)<\/span><\/td>\nBlasticidin-S deaminase ORF<\/span><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
3161-3483: (323)<\/span><\/td>\nT. brucei<\/em> \u03b1\u03b2 tubulin mRNA processing 3′<\/span><\/td>\nAdditional selectable markers<\/strong><\/span><\/td>\n<\/td>\n<\/tr>\n
3484-3728: (245)<\/span><\/td>\n6MYC<\/em> Expression Cassette<\/strong><\/span><\/td>\nHYG<\/em><\/a><\/span><\/td>\n6MYC<\/sup>x<\/span><\/td>\nSpe<\/em>I – Sac<\/em>I<\/span><\/td>\n<\/tr>\n
<\/td>\nHin<\/em>dIII Pac<\/em>I<\/span> ATG<\/strong> Xba<\/em>I — [cMYC<\/em>]6<\/sub> — Avr<\/em>II TGA<\/strong> Bam<\/em>HI<\/td>\nGFP<\/sup>x<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n
    1  GAATTCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT\n   51  ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT\n  101  TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT\n  151  CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG\n  201  CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG\n  251  GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT\n  301  GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC\n  351  TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC\n  401  CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA\n  451  GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC\n  501  GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT\n  551  TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG\n  601  GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG\n  651  AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG\n  701  CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT\n  751  CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG\n  801  CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC\n  851  TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG\n  901  TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA\n  951  TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG\n 1001  TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC\n 1051  GTTCATCCAT AGTTGCCTGA CTCCCCGTGG TGTAGATAAC TACGATACGG\n 1101  GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG\n 1151  CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG\n 1201  AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT\n 1251  TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA\n 1301  CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA\n 1351  TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC\n 1401  CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT\n 1451  CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC\n 1501  ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT\n 1551  GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG\n 1601  CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT\n 1651  TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG\n 1701  ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA\n 1751  CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA\n 1801  CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT\n 1851  TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG\n 1901  TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC\n 1951  AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA\n 2001  GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG\n 2051  GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA\n 2101  TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC\n 2151  AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG\n 2201  GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATTCGA\n 2251  CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT\n 2301  GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCAAGG AGATGGCGCC\n 2351  CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG\n 2401  CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG\n 2451  GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCTGG\n 2501  GTCCCATTGT TTGCCTCTTC CGCTGTGTGG AGTGCGCCTA CACGCACTTC\n 2551  TCACTTCGTA AGTGGTGGTG GCGTAAGTAT TGCCTAATGT TGACTCTATG\n 2601  TTCTCCTCTC CTCACCCCCT CGCGGTGCTG ATTTCTGACA GATCTTCAAA\n 2651  CACTAGTTTA AGCAAAGGAC TATTCATCCG TTTATATTAG CAACAGTAGG\n 2701  TACTAGCACC ACTAACAACA ACAACAAAGC ACTTCTGCAG GAATTCATGG\n 2751  CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT\n 2801  ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG CCAGCGCAGC\n 2851  TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA\n 2901  CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG\n 2951  GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG\n 3001  CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC\n 3051  CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT\n 3101  GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAGAATT\n 3151  CGAGCTCGCC CTAGAAAGTG TGACAACGTC GCACCATGTG TAGGTTTTCA\n 3201  TTTATGTTCT TTCTTTCTTT CTTTTGTGAA TTTGTTTTCT GTCTCAAATG\n 3251  TTTTTAATTC GCTTGGGACC TATGTTTTTC TTGTTTTTTT GCTCACCCTT\n 3301  TGTGTAGGAG GCACCCTGTC ACGTCTGTGG TTGCGTGTAT GCCTTCCTTC\n 3351  CCCTTATTCG CTTCTTCCTG TCGTGTCACA CCTCTTTCTC CCTCTCCCTT\n 3401  TCCGCCTTTT CTTTCAATCT TGTTTTCTCG ACCAGCCCTA CTAGAGGAGA\n 3451  AAGAATAGTA ACCCTTTCAT CAAAGAAAAT AGTAAGCTTA ATTAATGTCT\n 3501  AGAGAACAGA AACTGATCTC TGAAGAAGAC CTGCCTAGAG AACAGAAACT\n 3551  GATCTCTGAA GAAGACCTGC CTAGAGAACA GAAACTGATC TCTGAAGAAG\n 3601  ACCTGCCTAG AGAACAGAAA CTGATCTCTG AAGAAGACCT GCCTAGAGAA\n 3651  CAGAAACTGA TCTCTGAAGA AGACCTGCCT AGAGAACAGA AACTGATCTC\n 3701  TGAAGAAGAC CTGCCTAGGT GAGGATCC<\/pre>\n","protected":false},"excerpt":{"rendered":"
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