{"id":237,"date":"2012-10-16T15:32:41","date_gmt":"2012-10-16T14:32:41","guid":{"rendered":"http:\/\/blogs.lshtm.ac.uk\/alsfordlab\/?page_id=237"},"modified":"2025-11-24T09:52:40","modified_gmt":"2025-11-24T09:52:40","slug":"hygromycin-b-phosphotransferase","status":"publish","type":"page","link":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/pnat-tag-x\/hygromycin-b-phosphotransferase\/","title":{"rendered":"Hygromycin-B Phosphotransferase"},"content":{"rendered":"<p><span style=\"font-family: arial\">Derived from known sequence<\/span><\/p>\n<p><span style=\"font-family: arial\"><em>Spe<\/em>I &#8212; <em>HYG<\/em> &#8212; <em>Sac<\/em>I<\/span><\/p>\n<pre class=\"wp-block-preformatted\">    1  ACTAGTGATG AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC\n   51  TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC\n  101  GAAGAATCTC GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT\n  151  GCGGGTAAAT AGCTGCGCCG ATGGTTTCTA CAAAGATCGT TATGTTTATC\n  201  GGCACTTTGC ATCGGCCGCG CTCCCGATTC CGGAAGTGCT TGACATTGGG\n  251  GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG CACAGGGTGT\n  301  CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG\n  351  TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC\n  401  GGGTTCGGCC CATTCGGACC GCAAGGAATC GGTCAATACA CTACATGGCG\n  451  TGATTTCATA TGCGCGATTG CTGATCCCCA TGTGTATCAC TGGCAAACTG\n  501  TGATGGACGA CACCGTCAGT GCGTCCGTCG CGCAGGCTCT CGATGAGCTG\n  551  ATGCTTTGGG CCGAGGACTG CCCCGAAGTC CGGCACCTCG TGCACGCGGA\n  601  TTTCGGCTCC AACAATGTCC TGACGGACAA TGGCCGCATA ACAGCGGTCA\n  651  TTGACTGGAG CGAGGCGATG TTCGGGGATT CCCAATACGA GGTCGCCAAC\n  701  ATCTTCTTCT GGAGGCCGTG GTTGGCTTGT ATGGAGCAGC AGACGCGCTA\n  751  CTTCGAGCGG AGGCATCCGG AGCTTGCAGG ATCGCCGCGG CTCCGGGCGT\n  801  ATATGCTCCG CATTGGTCTT GACCAACTCT ATCAGAGCTT GGTTGACGGC\n  851  AATTTCGATG ATGCAGCTTG GGCGCAGGGT CGATGCGACG CAATCGTCCG\n  901  ATCCGGAGCC GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG\n  951  CCGTCTGGAC CGATGGCTGT GTAGAAGTAC TCGCCGATAG TGGAAACCGA\n 1001  CGCCCCAGCA CTCGTCCGAG GGCAAAGGAA TAGAGCTC<\/pre>\n","protected":false},"excerpt":{"rendered":"<p>Derived from known sequence SpeI &#8212; HYG &#8212; SacI 1 ACTAGTGATG AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC&#8230;<\/p>\n","protected":false},"author":116,"featured_media":0,"parent":226,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-237","page","type-page","status-publish","hentry","odd"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.9 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Hygromycin-B Phosphotransferase - Alsford Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/cell-lines-plasmids\/pnat-tag-x\/hygromycin-b-phosphotransferase\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Hygromycin-B Phosphotransferase - Alsford Lab\" \/>\n<meta property=\"og:description\" content=\"Derived from known sequence SpeI &#8212; 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