{"id":9,"date":"2012-06-15T14:47:14","date_gmt":"2012-06-15T13:47:14","guid":{"rendered":"http:\/\/blogs.lshtm.ac.uk\/alsfordlab\/?page_id=9"},"modified":"2025-11-24T09:45:46","modified_gmt":"2025-11-24T09:45:46","slug":"publications","status":"publish","type":"page","link":"https:\/\/blogs.lshtm.ac.uk\/alsfordlab\/publications\/","title":{"rendered":"Publications"},"content":{"rendered":"

Google Scholar <\/a> | ORCID iD: 0000-0001-8621-4920<\/a><\/p>\n

Bis-6-amidino-benzothiazole Derivative that Cures Experimental Stage 1 African Trypanosomiasis with a Single Dose.<\/strong>
Racan\u00e9 L, Pti\u010dek L, Kostrun S, Rai\u0107-Mali\u0107 S, Taylor MC, Delves M, Alsford S, Olmo F, Francisco Fortes, A, Kelly JM (2023) Journal of Medicinal Chemistry<\/em> 66: 13043-13057<\/a><\/p>\n

Synthetic biology tools for engineering Goodwin oscillation in Trypanosoma brucei.<\/strong>
Borg Y, Alsford S, Pavlika V, Zaikin A, Nesbeth DN (2022) Heliyon<\/em> 8:e08891<\/a><\/p>\n

TbSAP is a novel chromatin protein repressing metacyclic variant surface glycoprotein expression sites in bloodstream form Trypanosoma brucei<\/em>.<\/strong>
Davies C, Ooi CP, Sioutas G, Hall BS, Sidhu H, Butter F, Alsford S, Wickstead B, Rudenko G (2021) Nucleic Acids Research<\/em> 49: 3242-62<\/a><\/p>\n

2016-2020<\/h2>\n

Chemogenomic profiling of anti-leishmanial efficacy and resistance in the related kinetoplastid parasite Trypanosoma brucei<\/em>.<\/strong>
Collett CF, Kitson C, Baker N, Steele-Stallard HB, Santrot MV, Hutchinson S, Horn D, Alsford S (2019) Antimicrobial Agents & Chemotherapy<\/em> 63: e00795-19<\/a><\/p>\n

Insights into antitrypanosomal drug mode-of-action from cytology-based profiling.<\/strong>
Thomas J, Baker N, Glover L, Dominicus C, Alsford S, Hutchinson S, Horn D (2018) PLoS Neglected Tropical Diseases<\/em> 12: e0006980<\/a><\/p>\n

Decoding the network of Trypanosoma brucei<\/em> proteins that determines sensitivity to apolipoprotein-L1.<\/strong>
Currier RB, Cooper A, Burrell-Saward H, MacLeod A, Alsford S (2018) PLoS Pathogens<\/em> 14: e1006855<\/a><\/p>\n

Ornithine uptake and the modulation of drug sensitivity in Trypanosoma brucei<\/em>.<\/strong>
Macedo JP, Currier RB, Wirdnam C, Horn D, Alsford S, Rentsch D (2017) FASEB Journal<\/em> 31: 4649-60<\/a><\/p>\n

Distinct activation mechanisms trigger the trypanocidal activity of DNA damaging prodrugs.<\/strong>
Meredith E, Kumar A, Konno A, Szular J, Alsford S, Seifert K, Horn D, Wilkinson S (2017) Molecular Microbiology<\/em> 106: 207-22<\/a><\/p>\n

Genome-wide and protein kinase-focused RNAi screens reveal conserved and novel damage response pathways in Trypanosoma brucei<\/em>.<\/strong>
Stortz JA, Serafim TD, Alsford S, Wilkes J, Fernandes-Cortes F, Hamilton G, Briggs E, Lemgruber L, Horn D, Mottram JC, McCulloch R (2017) PLoS Pathogens<\/em> 13: e1006477<\/a><\/p>\n

Co-dependence between trypanosome nuclear lamina components in nuclear stability and control of gene expression.<\/strong>
Maishman L, Obado SO, Alsford S, Bart J, Chen W-M, Ratushny AV, Navarro M, Horn D, Aitchison JD, Chait BT, Rout MP, Field MC (2016) Nucleic Acids Research<\/em> 44: 10554-70<\/a><\/p>\n

VEX1 controls the allelic exclusion required for antigenic variation in trypanosomes.<\/strong>
Glover L, Hutchinson S, Alsford S, Horn D (2016) Proceedings of the National Academy of Sciences (USA)<\/em> 113: 7225-30<\/a><\/p>\n

2011-15<\/h2>\n

Modulation of the surface proteome through multiple ubiquitylation pathways in African trypanosomes.<\/strong>
Zoltner M, Leung K-F, Alsford S, Horn D, Field MC (2015) PLoS Pathogens<\/em> 11: e1005236<\/a><\/p>\n

Genome-scale RNAi screens for high-throughput phenotyping in bloodstream form African trypanosomes.<\/strong>
Glover L, Alsford S, Baker N, Turner DJ, Sanchez-Flores A, Hutchinson S, Hertz-Fowler C, Berriman M and Horn D (2015) Nature Protocols<\/em> 10: 106-33<\/a><\/p>\n

Subverting lysosomal function in Trypanosoma<\/em><\/strong><\/b> brucei<\/em> <\/strong>(Editorial)
Alsford S (2015) Microbial Cell<\/em> 2: 259-61<\/a><\/p>\n

Increased Trypanosoma brucei<\/em> cathepsin-L activity inhibits human serum-mediated trypanolysis<\/strong><\/b> (Commentary)
Alsford S (2014) Microbial Cell<\/em>1: 270-2<\/a><\/p>\n

Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei<\/i>.<\/b>
Alsford S, Currier RB, Guerra-Assuncao JA, Clark TG, Horn D (2014)\u00a0PLoS Pathogens<\/em>\u00a0 10: e1004130<\/a><\/p>\n

Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNAi library screening for resistance to the phosphodiesterase inhibitor Cpd A.<\/strong>
Gould M, Bachmaier S, Ali J, Alsford S, Tagoe D, Munday J, Schnauffer A, Horn D, Boshart M, De Koning H (2013) Antimicrobial Agents & Chemotherapy<\/em> 57: 4882-93<\/a><\/p>\n

DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes.<\/strong>
Glover L, Alsford S, Horn D (2013) PLoS Pathogens<\/em>\u00a09: e1003260<\/a><\/p>\n

Receptor mediated endocytosis for drug delivery in African trypanosomes: fulfilling Paul Ehrlich\u2019s vision of chemotherapy <\/strong>(Review)
Alsford S, Field MC, Horn D (2013) Trends in Parasitology<\/em> 29: 207-12<\/a>
(Featured on the front cover of the May<\/a> issue)<\/p>\n

Antigenic variation in African trypanosomes: the importance of chromosomal and nuclear context in VSG expression control<\/strong> (Review)
Glover L, Hutchinson S, Alsford S, McCulloch R, Field MC, Horn D (2013) Cellular Microbiology<\/em> 15: 1984-93<\/a> | PDF<\/a><\/p>\n

Genetic dissection of drug resistance in trypanosomes<\/strong> (Review)
Alsford S, Kelly JM, Baker N, Horn D (2013) Parasitology<\/em> 140: 1478-91<\/a> | PDF<\/a><\/p>\n

Cell cycle regulated control of VSG expression site silencing by the chromatin chaperones CAF-1b and ASF1A in T. brucei<\/em>.<\/strong>
Alsford S, Horn D (2012) Nucleic Acids Research<\/em> 40: 10150-60<\/a><\/p>\n

NUP-1 is a large coiled-coil nucleoskeletal protein in trypanosomes with lamin-like functions.<\/strong>
DuBois K, Alsford S, Holden JM, Buisson J, Swiderski M, Bart J, Bastin P, Barry D, Navarro M, Horn D, Aitchison J, Rout M, Field MC (2012) PLoS Biology<\/em> 10: e1001287<\/a><\/p>\n

High-throughput decoding of anti-trypanosomal drug efficacy and resistance.<\/strong>
Alsford S, Eckert S, Baker N, Glover L, Sanchez-Flores A, Leung K-F, Turner DJ, Field MC, Berriman M, Horn D (2012) Nature<\/em>, 482: 232-6<\/a> | PDF<\/a><\/p>\n

Telomeres, tethers and trypanosomes <\/strong>(Review)
Field MC, Horn D, Alsford S, Koreny L, Rout MP (2012) Nucleus<\/em> 3: 478-86<\/a> | PDF<\/a><\/p>\n

Epigenetic mechanisms, nuclear architecture and control of gene expression in trypanosomes<\/strong> (Review)
Alsford S, DuBois K, Horn D, Field MC (2012) Expert Reviews in Molecular Medicine<\/em> 14: e13<\/a><\/p>\n

Elongator Protein 3b Negatively Regulates Ribosomal RNA Transcription in African Trypanosomes.<\/strong>
Alsford S, Horn D (2011) Molecular & Cellular Biology<\/em> 31: 1822-32<\/a> | PDF<\/a><\/p>\n

High throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome.<\/strong>
Alsford S, Turner DJ, Obado SO, Sanchez-Flores A, Glover L, Berriman M, Hertz-Fowler C, Horn D (2011) Genome Research <\/em>21: 915-24<\/a> | PDF<\/a><\/p>\n

Genome-wide RNAi screens in African trypanosomes identify the nifurtimox activator NTR and the eflornithine transporter AAT6.<\/strong>
Baker N, Alsford S, Horn D (2011) Molecular & Biochemical Parasitology<\/em> 176: 55-7<\/a> | PDF<\/a><\/p>\n

2006-2010<\/h2>\n

RNA interference, growth and differentiation appear normal in African trypanosomes lacking Tudor staphylococcal nuclease.<\/strong>
Alsford S, Kemp L, Kawahara T, Horn D (2010) Molecular & Biochemical Parasitology<\/em> 174: 70-3<\/a> | PDF<\/a><\/p>\n

DNA breaks as triggers for antigenic variation in African trypanosomes<\/strong><\/strong> (Review)
Alsford S, Horn D, Glover L (2009) Genome Biology <\/em>10: 223<\/a> | PDF<\/a><\/p>\n

Two essential MYST-family proteins display distinct roles in histone H4K10 acetylation and telomeric silencing in trypanosomes. <\/strong><\/strong>
Kawahara T, Siegel TN, Ingram AK, Alsford S, Cross GA, Horn D (2008) Molecular Microbiology<\/em> 69: 1054-68<\/a> | PDF<\/a><\/p>\n

Single-locus targeting constructs for reliable regulated RNAi and trans<\/em>-gene expression in Trypanosoma brucei<\/em>.<\/strong>
Alsford S, Horn D (2008) Molecular & Biochemical Parasitology<\/em> 161: 76-9<\/a><\/p>\n

RNA polymerase I transcription stimulates homologous recombination in Trypanosoma brucei<\/em>.<\/strong>
Alsford S, Horn D (2007) Molecular & Biochemical Parasitology<\/em> 153:77-9<\/a><\/p>\n

Deletion of a trypanosome telomere leads to loss of silencing and progressive loss of terminal DNA in the absence of cell cycle arrest.<\/strong>
Glover L, Alsford S, Beattie C, Horn D (2007) Nucleic Acids Research<\/em> 35: 872-80<\/a> | PDF<\/a><\/p>\n

A sirtuin in the African trypanosome is involved in both DNA repair and telomeric gene silencing but is not required for antigenic variation.<\/strong>
Alsford S, Kawahara T, Isamah C, Horn D (2007) Molecular Microbiology<\/em> 63: 724-36<\/a> | PDF<\/a><\/p>\n

2001-2005<\/h2>\n

Tagging a T. brucei<\/em> RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects.<\/strong>
Alsford S, Kawahara T, Glover L, Horn D (2005) Molecular & Biochemical Parasitology<\/em> 144: 142-8<\/a><\/p>\n

Multiplex analysis of RNA interference defects in T. brucei<\/em>.<\/strong>
Alsford S, Glover L, Horn D. (2005) Molecular & Biochemical Parasitology<\/em> 139: 129-32<\/a><\/p>\n

Trypanosomatid histones<\/strong> (Review)
Alsford S, Horn D (2004)  Molecular Microbiology<\/em> 53: 365-372<\/a> | PDF<\/a><\/p>\n

The identification of circular extrachromosomal DNA in the nuclear genome of T. brucei<\/em>.<\/strong>
Alsford NS, Navarro M, Jamnadass HR, Dunbar H, Ackroyd M, Murphy NB, Gull K, Ersfeld K (2003) Molecular Microbiology<\/em> 47: 277-89<\/a> | PDF<\/a><\/p>\n

Diversity and dynamics of the minichromosomal karyotype in T. brucei<\/em>.<\/strong>
Alsford S, Wickstead B, Ersfeld K, Gull K (2001) Molecular & Biochemical Parasitology<\/em> 113: 79-88<\/a><\/p>\n

1995-2000<\/h2>\n

Segregation of minichromosomes in trypanosomes: implications for mitotic mechanisms (Review)<\/strong>
Gull K, Alsford S, Ersfeld K (1998) Trends in Microbiology<\/em> 6: 319-23<\/a><\/p>\n

A fluorescence in situ hybridisation study of the regulation of histone mRNA levels during the cell cycle of T. brucei<\/em>.<\/strong>
Ersfeld K, Docherty R, Alsford S, Gull K (1996) Molecular & Biochemical Parasitology<\/em> 30: 201-9<\/a><\/p>\n

Molecular forms of tegumental and muscle acetylcholinesterases of Schistosoma<\/em>.<\/strong>
Camacho M, Alsford S, Agnew A (1996) Parasitology<\/em> 112: 199-204<\/a><\/p>\n

Nicotinic acetylcholine receptors on the surface of the blood fluke Schistosoma<\/em>.<\/strong>
Camacho M, Alsford S, Jones A, Agnew A (1995) Molecular & Biochemical Parasitology<\/em> 71: 127-34<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

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