pNAT tag-X
Derived from known sequence
| Landmarks | Available expression cassettes | |||
| 0001-2497: (2497) | Vector backbone | Sequence page | Expression cassette | Region replaced |
| 2498-2736: (239) | T. brucei βα tubulin mRNA processing 5′ | N-terminal eGFP | GFPx | HindIII – BamHI |
| 2747-3145: (399) | Blasticidin-S deaminase ORF | |||
| 3161-3483: (323) | T. brucei αβ tubulin mRNA processing 3′ | Additional selectable markers | ||
| 3484-3728: (245) | 6MYC Expression Cassette | HYG | 6MYCx | SpeI – SacI |
| HindIII PacI ATG XbaI — [cMYC]6 — AvrII TGA BamHI | GFPx | |||
1 GAATTCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT 51 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 101 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 151 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 201 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 251 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 301 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 351 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 401 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 451 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 501 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 551 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 601 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 651 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 701 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 751 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 801 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 851 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 901 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 951 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 1001 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 1051 GTTCATCCAT AGTTGCCTGA CTCCCCGTGG TGTAGATAAC TACGATACGG 1101 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 1151 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 1201 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 1251 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 1301 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 1351 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 1401 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 1451 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 1501 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 1551 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 1601 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 1651 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 1701 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 1751 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 1801 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 1851 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 1901 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 1951 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA 2001 GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG 2051 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 2101 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC 2151 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 2201 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATTCGA 2251 CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT 2301 GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCAAGG AGATGGCGCC 2351 CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG 2401 CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG 2451 GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCTGG 2501 GTCCCATTGT TTGCCTCTTC CGCTGTGTGG AGTGCGCCTA CACGCACTTC 2551 TCACTTCGTA AGTGGTGGTG GCGTAAGTAT TGCCTAATGT TGACTCTATG 2601 TTCTCCTCTC CTCACCCCCT CGCGGTGCTG ATTTCTGACA GATCTTCAAA 2651 CACTAGTTTA AGCAAAGGAC TATTCATCCG TTTATATTAG CAACAGTAGG 2701 TACTAGCACC ACTAACAACA ACAACAAAGC ACTTCTGCAG GAATTCATGG 2751 CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT 2801 ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG CCAGCGCAGC 2851 TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA 2901 CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG 2951 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG 3001 CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC 3051 CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT 3101 GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAGAATT 3151 CGAGCTCGCC CTAGAAAGTG TGACAACGTC GCACCATGTG TAGGTTTTCA 3201 TTTATGTTCT TTCTTTCTTT CTTTTGTGAA TTTGTTTTCT GTCTCAAATG 3251 TTTTTAATTC GCTTGGGACC TATGTTTTTC TTGTTTTTTT GCTCACCCTT 3301 TGTGTAGGAG GCACCCTGTC ACGTCTGTGG TTGCGTGTAT GCCTTCCTTC 3351 CCCTTATTCG CTTCTTCCTG TCGTGTCACA CCTCTTTCTC CCTCTCCCTT 3401 TCCGCCTTTT CTTTCAATCT TGTTTTCTCG ACCAGCCCTA CTAGAGGAGA 3451 AAGAATAGTA ACCCTTTCAT CAAAGAAAAT AGTAAGCTTA ATTAATGTCT 3501 AGAGAACAGA AACTGATCTC TGAAGAAGAC CTGCCTAGAG AACAGAAACT 3551 GATCTCTGAA GAAGACCTGC CTAGAGAACA GAAACTGATC TCTGAAGAAG 3601 ACCTGCCTAG AGAACAGAAA CTGATCTCTG AAGAAGACCT GCCTAGAGAA 3651 CAGAAACTGA TCTCTGAAGA AGACCTGCCT AGAGAACAGA AACTGATCTC 3701 TGAAGAAGAC CTGCCTAGGT GAGGATCC