Sam Alsford Bloodstream form T brucei RME LSHTM - as seen from Malet Street

Welcome to the Alsford lab web site…

…where you can find out about our research on the kinetoplastid parasites, Trypanosoma brucei and Leishmania. Work in the lab
focuses on two aspects of kinetoplastid biology. How these parasites interact with their host environment, especially the uptake
and sub-cellular transit of host- and parasite-derived molecules, including nutrients, drugs and innate immune factors. In addition,
we have a longstanding interest in the regulation of RNA polymerase-I transcription, specifically the intersection
between rRNA and VSG transcription in T. brucei.

Recent research…

Our longstanding collaboration with Prof Gloria Rudenko’s team at Imperial College led to the identification of TbSAP a protein
with a role in metacyclic VSG expression regulation. This work was published in the April 2021 edition of
Nucleic Acids Research.

Our work exploiting the T. brucei RNAi library to identify candidate anti-leishmanial drug efficacy determinants was published
in the August 2019 edition of Antimicrobial Agents & Chemotherapy (after a few months on BioRxiv – our first pre-print!).
We describe 42 novel drug efficacy determinants, all of which have orthologues in
Leishmania, including several proteins involved
in membrane trafficking and organisation.

Rachel’s paper describing the parasite factors that drive apolipoprotein-L1 action against T. brucei is available at PLoS Pathogens
– highlighting the importance of ubiquitination and membrane trafficking to apoL1 action.

If you’re interested in using the bloodstream-form T. brucei RNAi library in your research, or adapting the RIT-seq approach to
your preferred organism
, extensive detailed protocols are available at Nature Protocols.

Explore our other pages…

…to find details of our cell lines & plasmids (all freely available to the research community on request), protocols and publications.

There are also extensive links to other online resources and Trypanosomatid research groups based in the UK and beyond. If your lab
is missing from this list (apologies!) or the link is out of date, drop me an email, and I’ll be sure to update the list.

Read our Blog for intermittent postings on publications, research, conferences, and public engagement activities.

|  T: 020 7927 2699(lab) 020 7927 2352(office)  |  Lab333, LSHTM, Keppel Street, London WC1E 7HT.

Department of Infection Biology  |  Infectious & Tropical Diseases  |  LSHTM