pNAT X-tag

Derived from known sequence

Landmarks Additional expression cassettes
0001-1535: (1535) eGFP Expression cassette Sequence page Expression cassette Region replaced
AscI HindIII — 792bp stuffer — XbaI — eGFP STOP BamHI C-terminal 12MYC x12MYC XbaI – BamHI
1536-2166: (631) T. brucei βα tubulin mRNA processing 5′
2180-3205: (1026) Hygromycin-B phosphotransferase ORF
3209-3498: (290) T. brucei actin mRNA processing 3′ Additional selectable markers
3499-6034: (2536) Vector backbone BSD xeGFP KpnI – StuI
x12MYC
    1  GGCGCGCCAA GCTTNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
   51  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  601  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  651  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  701  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
  801  NNNNNNTCTA GAGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
  851  CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
  901  CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC
  951  ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
 1001  CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
 1051  AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
 1101  ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA
 1151  GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
 1201  TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC
 1251  AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
 1301  GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
 1351  ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC
 1401  GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
 1451  GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
 1501  CTCTCGGCAT GGACGAGCTG TACAAGTGAG GATCCACGCG GACGGGGCAT
 1551  TTCCCGTTCG TCATTAGCAG TAGGTAATGA AGATGTTTGT TTCTCGTCCC
 1601  CTTTCTCCTT CGTCCTTCTG TCATTTTGTT CTTTTGTGTT TATGTTTTGT
 1651  TGTTGTTTTC TTTAATTTTT TTTTTCTTCC ACGTTTGTGT ACATCCGCGC
 1701  GCCACTCTAT TCAGAGAGCC ACGGATAGTA GAGGAGGTGG GAAGGGTATA
 1751  TGAGGGACAC GCGTACCATG ATGTGGGATG TATTGGGGTC CCTGTCTGTC
 1801  CTTACGTGAC TATGTATGAA CCGTCACGTG TAAGATGAGC TAGTGAGATC
 1851  AACAGTACAA CTCATCAACA CGCCTTCTTC TCGTTAAATG TACACAATCT
 1901  TGATCCTCCA CCTTTATGGG TCCCATTGTT TGCCTCTTCC GCTGTGTGGA
 1951  GTGCGCCTAC ACGCACTTCT CACTTCGTAA GTGGTGGTGG CGTAAGTATT
 2001  GCCTAATGTT GACTCTATAT TCTCCTCTCC TCACCCCCTC GCGGTGCTGA
 2051  TTTCTGACAG ATCTTCAAAC ACTAGTTTAA GCAAAGGACT ATTCATCCGT
 2101  TTATATTAGC AACAGTAGGT ACTAGCACCA CTAACAACAA CAACAAAGCA
 2151  CTTCTATTTA TTTATCGGTA CCACTAGTGA TGAAAAAGCC TGAACTCACC
 2201  GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA
 2251  CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG
 2301  TAGGAGGGCG TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC
 2351  TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT
 2401  TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG ACCTATTGCA
 2451  TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA
 2501  CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC
 2551  GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA
 2601  TCGGTCAATA CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC
 2651  CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT
 2701  CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG
 2751  TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC
 2801  AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA
 2851  TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT
 2901  GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA
 2951  GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT
 3001  CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG
 3051  GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA
 3101  CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT
 3151  ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
 3201  AATAGAGGCC TCGGAGATCC TAACACCGGG TTGTGTGGCC AAAATTGTTC
 3251  TGTAGTCGCT GTGAGTTGAC ACGGCTAGTG CTTATGATTT TCCTCGCGTG
 3301  TGGTGCCTGT ACTCAGCCCT ATGCCTTATT TGCAACACAT TTACGTACAG
 3351  CGCACAAGAG AAGAGAAGAT CACTTGAAGA TAATAAATAT AGGGTTGTAG
 3401  GCATCTTGTT TAACTCAAAT TTTCTCGTCT TGGTGTGTCG ACATGATTGA
 3451  AATAGTGCCA CCAGTTGTGT TTGATGCGTT TGTTATCTAT GCAGTATTCC
 3501  TAGCCAGATC CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA
 3551  CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT
 3601  CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT
 3651  GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCACCATT
 3701  CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA CCTACTACTG GGCTGCTTCC
 3751  TAATGCAGGA GTCGCATAAG GGAGAGCGTC GAATGGTGCA CTCTCAGTAC
 3801  AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC
 3851  CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA
 3901  CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC
 3951  ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT
 4001  AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT
 4051  CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC
 4101  AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT
 4151  ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT
 4201  CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
 4251  GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA
 4301  TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA
 4351  GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT
 4401  ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT
 4451  ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT
 4501  ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
 4551  TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG
 4601  AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT
 4651  CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC
 4701  CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG
 4751  AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG
 4801  GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT
 4851  TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG
 4901  CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG
 4951  ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT
 5001  AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT
 5051  ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG
 5101  GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT
 5151  TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
 5201  GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA
 5251  CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT
 5301  TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC
 5351  TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
 5401  ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
 5451  TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
 5501  CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG
 5551  CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG
 5601  CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA
 5651  GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
 5701  TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
 5751  TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
 5801  CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC
 5851  TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA
 5901  GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG
 5951  TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
 6001  CGTTGGCCGA TTCATTAATG CAGAATTCGA GCTC