Protocols

[Under construction]

A selection of Trypanosoma brucei-specific protocols, updated on an ad hoc basis; for details of standard molecular biology protocols see, for example, Current Protocols in Molecular Biology or Short Protocols in Molecular Biology, both edited by Ausubel et al.

Trypanosoma brucei culture & manipulation

Trypanosoma brucei growth analysis

  • Basic growth assay (& calculating a doubling time)
  • EC50 analysis (calculating a ‘half maximal effective growth-inhibitory concentration’)
  • Isobologram analysis (synergistic, additive & antagonistic inhibitor interactions)

Molecular Biology

Cell biology

Protein analysis

RNA analysis