Publications

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2017 (*joint corresponding author)
Ornithine uptake and the modulation of drug sensitivity in Trypanosoma brucei.
Macedo JP, Currier RB, Wirdnam C, Horn D, Alsford S*, Rentsch D* (2017) FASEB Journal 31: 4649-60
Distinct activation mechanisms trigger the trypanocidal activity of DNA damaging prodrugs.
Meredith E, Kumar A, Konno A, Szular J, Alsford S, Seifert K, Horn D, Wilkinson S (2017) Molecular Microbiology (in press)
Genome-wide and protein kinase-focused RNAi screens reveal conserved and novel damage response pathways in Trypanosoma brucei.
Stortz JA, Serafim TD, Alsford S, Wilkes J, Fernandes-Cortes F, Hamilton G, Briggs E, Lemgruber L, Horn D, Mottram JC, McCulloch R (2017) PLoS Pathogens 13: e1006477

2016
Co-dependence between trypanosome nuclear lamina components in nuclear stability and control of gene expression.
Maishman L, Obado SO, Alsford S, Bart J, Chen W-M, Ratushny AV, Navarro M, Horn D, Aitchison JD, Chait BT, Rout MP, Field MC (2016) Nucleic Acids Research 44: 10554-70
VEX1 controls the allelic exclusion required for antigenic variation in trypanosomes.
Glover L, Hutchinson S, Alsford S, Horn D (2016) Proceedings of the National Academy of Sciences (USA) 113: 7225-30

2015
Modulation of the surface proteome through multiple ubiquitylation pathways in African trypanosomes.
Zoltner M, Leung K-F, Alsford S, Horn D, Field MC (2015) PLoS Pathogens 11: e1005236
Genome-scale RNAi screens for high-throughput phenotyping in bloodstream form African trypanosomes.
Glover L, Alsford S, Baker N, Turner DJ, Sanchez-Flores A, Hutchinson S, Hertz-Fowler C, Berriman M and Horn D (2015) Nature Protocols 10: 106-33

Editorial
Subverting lysosomal function in Trypanosoma brucei.
Alsford S (2015) Microbial Cell 2: 259-61

2014 (*corresponding author)
Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei.
*Alsford S, Currier RB, Guerra-Assuncao JA, Clark TG, Horn D (2014) PLoS Pathogens 10: e1004130

Commentary
Increased Trypanosoma brucei cathepsin-L activity inhibits human serum-mediated trypanolysis.
Alsford S (2014) Microbial Cell 1: 270-2

2013 (*corresponding author)
Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNAi library screening for resistance to the phosphodiesterase inhibitor Cpd A.
Gould M, Bachmaier S, Ali J, Alsford S, Tagoe D, Munday J, Schnauffer A, Horn D, Boshart M, De Koning H (2013) Antimicrobial Agents & Chemotherapy 57: 4882-93
DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes.
Glover L, Alsford S, Horn D (2013) PLoS Pathogens 9: e1003260

Opinion
Receptor mediated endocytosis for drug delivery in African trypanosomes: fulfilling Paul Ehrlich’s vision of chemotherapy.
*Alsford S, Field MC, Horn D (2013) Trends in Parasitology 29: 207-12
(Featured on the front cover of the May issue)

Reviews
Antigenic variation in African trypanosomes: the importance of chromosomal and nuclear context in VSG expression control.
Glover L, Hutchinson S, Alsford S, McCulloch R, Field MC, Horn D (2013) Cellular Microbiology 15: 1984-93 | PDF
Genetic dissection of drug resistance in trypanosomes.
*Alsford S, Kelly JM, Baker N, Horn D (2013) Parasitology 140: 1478-91 | PDF

2012
Cell cycle regulated control of VSG expression site silencing by the chromatin chaperones CAF-1b and ASF1A in T. brucei.
Alsford S, Horn D (2012) Nucleic Acids Research 40: 10150-60
Identification of a coiled-coil lamin-like protein in T. brucei.
DuBois K, Alsford S, Holden JM, Buisson J, Swiderski M, Bart J, Bastin P, Barry D, Navarro M, Horn D, Aitchison J, Rout M, Field MC (2012) PLoS Biology 10: e1001287
High-throughput decoding of anti-trypanosomal drug efficacy and resistance.
Alsford S, Eckert S, Baker N, Glover L, Sanchez-Flores A, Leung K-F, Turner DJ, Field MC, Berriman M, Horn D (2012) Nature, 482: 232-6 | PDF

Extraview
Telomeres, tethers and trypanosomes.
Field MC, Horn D, Alsford S, Koreny L, Rout MP (2012) Nucleus 3: 478-86 | PDF

Review
Epigenetic mechanisms, nuclear architecture and control of gene expression in trypanosomes.
Alsford S, DuBois K, Horn D, Field MC (2012) Expert Reviews in Molecular Medicine 14: e13

2011
Elongator Protein 3b Negatively Regulates Ribosomal RNA Transcription in African Trypanosomes.
Alsford S, Horn D (2011) Molecular & Cellular Biology 31: 1822-32 | PDF
High throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome.
Alsford S, Turner DJ, Obado SO, Sanchez-Flores A, Glover L, Berriman M, Hertz-Fowler C, Horn D (2011) Genome Research 21: 915-24 | PDF
Genome-wide RNAi screens in African trypanosomes identify the nifurtimox activator NTR and the eflornithine transporter AAT6.
Baker N, Alsford S, Horn D (2011) Molecular & Biochemical Parasitology 176: 55-7 | PDF

2006-2010
RNA interference, growth and differentiation appear normal in African trypanosomes lacking Tudor staphylococcal nuclease.
Alsford S, Kemp L, Kawahara T, Horn D (2010) Molecular & Biochemical Parasitology 174: 70-3 | PDF
Two essential MYST-family proteins display distinct roles in histone H4K10 acetylation and telomeric silencing in trypanosomes.
Kawahara T, Siegel TN, Ingram AK, Alsford S, Cross GA, Horn D (2008) Molecular Microbiology 69: 1054-68 | PDF
Single-locus targeting constructs for reliable regulated RNAi and trans-gene expression in Trypanosoma brucei.
Alsford S, Horn D (2008) Molecular & Biochemical Parasitology 161: 76-9
RNA polymerase I transcription stimulates homologous recombination in Trypanosoma brucei.
Alsford S, Horn D (2007) Molecular & Biochemical Parasitology 153:77-9
Deletion of a trypanosome telomere leads to loss of silencing and progressive loss of terminal DNA in the absence of cell cycle arrest.
Glover L, Alsford S, Beattie C, Horn D (2007) Nucleic Acids Research 35: 872-80 | PDF
A sirtuin in the African trypanosome is involved in both DNA repair and telomeric gene silencing but is not required for antigenic variation.
Alsford S, Kawahara T, Isamah C, Horn D (2007) Molecular Microbiology 63: 724-36 | PDF

Minireview
DNA breaks as triggers for antigenic variation in African trypanosomes.
Alsford S, Horn D, Glover L (2009) Genome Biology 10: 223 | PDF

2001-2005
Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects.
Alsford S, Kawahara T, Glover L, Horn D (2005) Molecular & Biochemical Parasitology 144: 142-8
Multiplex analysis of RNA interference defects in T. brucei.
Alsford S, Glover L, Horn D. (2005) Molecular & Biochemical Parasitology 139: 129-32
The identification of circular extrachromosomal DNA in the nuclear genome of T. brucei.
Alsford NS, Navarro M, Jamnadass HR, Dunbar H, Ackroyd M, Murphy NB, Gull K, Ersfeld K (2003) Molecular Microbiology 47: 277-89 | PDF
Diversity and dynamics of the minichromosomal karyotype in T. brucei.
Alsford S, Wickstead B, Ersfeld K, Gull K (2001) Molecular & Biochemical Parasitology 113: 79-88

Review
Trypanosomatid histones.
Alsford S, Horn D (2004)  Molecular Microbiology 53: 365-372 | PDF

1995-2000
A fluorescence in situ hybridisation study of the regulation of histone mRNA levels during the cell cycle of T. brucei.
Ersfeld K, Docherty R, Alsford S, Gull K (1996) Molecular & Biochemical Parasitology 30: 201-9
Molecular forms of tegumental and muscle acetylcholinesterases of Schistosoma.
Camacho M, Alsford S, Agnew A (1996) Parasitology 112: 199-204
Nicotinic acetylcholine receptors on the surface of the blood fluke Schistosoma.
Camacho M, Alsford S, Jones A, Agnew A (1995) Molecular & Biochemical Parasitology 71: 127-34

Review
Segregation of minichromosomes in trypanosomes: implications for mitotic mechanisms.
Gull K, Alsford S, Ersfeld K (1998) Trends in Microbiology 6: 319-23