pNAT X-tag
Derived from known sequence
| Landmarks | Additional expression cassettes | |||
| 0001-1535: (1535) | eGFP Expression cassette | Sequence page | Expression cassette | Region replaced |
| AscI HindIII — 792bp stuffer — XbaI — eGFP — STOP BamHI | C-terminal 12MYC | x12MYC | XbaI – BamHI | |
| 1536-2166: (631) | T. brucei βα tubulin mRNA processing 5′ | |||
| 2180-3205: (1026) | Hygromycin-B phosphotransferase ORF | |||
| 3209-3498: (290) | T. brucei actin mRNA processing 3′ | Additional selectable markers | ||
| 3499-6034: (2536) | Vector backbone | BSD | xeGFP | KpnI – StuI |
| x12MYC | ||||
1 GGCGCGCCAA GCTTNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 51 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 101 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 151 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 201 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 251 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 301 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 351 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 601 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 651 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 701 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 751 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 801 NNNNNNTCTA GAGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC 851 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT 901 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 951 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC 1001 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC 1051 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC 1101 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA 1151 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT 1201 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC 1251 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT 1301 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG 1351 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC 1401 GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA 1451 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA 1501 CTCTCGGCAT GGACGAGCTG TACAAGTGAG GATCCACGCG GACGGGGCAT 1551 TTCCCGTTCG TCATTAGCAG TAGGTAATGA AGATGTTTGT TTCTCGTCCC 1601 CTTTCTCCTT CGTCCTTCTG TCATTTTGTT CTTTTGTGTT TATGTTTTGT 1651 TGTTGTTTTC TTTAATTTTT TTTTTCTTCC ACGTTTGTGT ACATCCGCGC 1701 GCCACTCTAT TCAGAGAGCC ACGGATAGTA GAGGAGGTGG GAAGGGTATA 1751 TGAGGGACAC GCGTACCATG ATGTGGGATG TATTGGGGTC CCTGTCTGTC 1801 CTTACGTGAC TATGTATGAA CCGTCACGTG TAAGATGAGC TAGTGAGATC 1851 AACAGTACAA CTCATCAACA CGCCTTCTTC TCGTTAAATG TACACAATCT 1901 TGATCCTCCA CCTTTATGGG TCCCATTGTT TGCCTCTTCC GCTGTGTGGA 1951 GTGCGCCTAC ACGCACTTCT CACTTCGTAA GTGGTGGTGG CGTAAGTATT 2001 GCCTAATGTT GACTCTATAT TCTCCTCTCC TCACCCCCTC GCGGTGCTGA 2051 TTTCTGACAG ATCTTCAAAC ACTAGTTTAA GCAAAGGACT ATTCATCCGT 2101 TTATATTAGC AACAGTAGGT ACTAGCACCA CTAACAACAA CAACAAAGCA 2151 CTTCTATTTA TTTATCGGTA CCACTAGTGA TGAAAAAGCC TGAACTCACC 2201 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA 2251 CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG 2301 TAGGAGGGCG TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC 2351 TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT 2401 TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG ACCTATTGCA 2451 TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA 2501 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC 2551 GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA 2601 TCGGTCAATA CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC 2651 CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT 2701 CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG 2751 TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC 2801 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA 2851 TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT 2901 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA 2951 GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT 3001 CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG 3051 GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA 3101 CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT 3151 ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG 3201 AATAGAGGCC TCGGAGATCC TAACACCGGG TTGTGTGGCC AAAATTGTTC 3251 TGTAGTCGCT GTGAGTTGAC ACGGCTAGTG CTTATGATTT TCCTCGCGTG 3301 TGGTGCCTGT ACTCAGCCCT ATGCCTTATT TGCAACACAT TTACGTACAG 3351 CGCACAAGAG AAGAGAAGAT CACTTGAAGA TAATAAATAT AGGGTTGTAG 3401 GCATCTTGTT TAACTCAAAT TTTCTCGTCT TGGTGTGTCG ACATGATTGA 3451 AATAGTGCCA CCAGTTGTGT TTGATGCGTT TGTTATCTAT GCAGTATTCC 3501 TAGCCAGATC CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA 3551 CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT 3601 CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT 3651 GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCACCATT 3701 CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA CCTACTACTG GGCTGCTTCC 3751 TAATGCAGGA GTCGCATAAG GGAGAGCGTC GAATGGTGCA CTCTCAGTAC 3801 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC 3851 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA 3901 CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC 3951 ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT 4001 AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT 4051 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 4101 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT 4151 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 4201 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 4251 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA 4301 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA 4351 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 4401 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT 4451 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 4501 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 4551 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG 4601 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT 4651 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 4701 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG 4751 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 4801 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 4851 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG 4901 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG 4951 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 5001 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT 5051 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 5101 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 5151 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 5201 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 5251 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 5301 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC 5351 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 5401 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 5451 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 5501 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 5551 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 5601 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA 5651 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 5701 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 5751 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 5801 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 5851 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 5901 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG 5951 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 6001 CGTTGGCCGA TTCATTAATG CAGAATTCGA GCTC