pRP series
Members of this plasmid series integrate at a random (non-transcribed) rDNA spacer locus following NotI linearisation in the RRNA targeting sequence.
Derived from known sequence and junction sequencing, February 2008.
| Landmarks | Available expression cassettes | |||
| 0001-0592: (592) | RRNA targeting fragment NotI | Sequence page | Expression cassette | Region replaced |
| 0593-3116: (2525) | Vector backbone (pUC/pGEM) | N-term eGFP | GFPx | HindIII – BamHI |
| 3117-3412: (296) | T. brucei actin mRNA processing 3’ | N-term MYC | MYCx | |
| 3413-4435: (1023) | Hygromycin B phosphotransferase ORF | N/C-term 6MYC | x6MYCx | |
| 4436-4693: (258) | T. brucei aldolase mRNA processing 5’ | N-term tdTomato | TOMx | |
| 4680-4913: (234) | EP procyclin promoter | C-term eGFP | xGFP | |
| 4919-5178: (260) | RRNA promoter | Stem-loop RNAi | MCS1/2 | Acc65I – Bsp120I |
| 5182-5194: (13) | Tet operator | Additional selectable markers | ||
| 5198-5292: (95) | T. brucei procyclin mRNA processing 5′ | BSD | MYCx | NgoMIV – BstZ17I |
| 5293-6747: (1455) | Expression Cassette (GFPx = 1,455 bp) | x6MYCx | ||
| 6748-7485: (738) | T. brucei aldolase mRNA processing 3’ | xGFP | ||
| 7486-7887: (402) | T7 terminator x2 fragment | xGFP constitutive | ||
1 GAATTCGATG CGAGGCGAAT CGCTCAGTAG TGTTAGTGCA GGCATTGTTT 51 CTCTTTTCCA TTTCGGCTCG CTCTCTGTGC CAGAAAGGCT TTTGGAGTCT 101 TGGTTACTCG TGGGACTAGG GCAAGCGTCG CTTCCCTAAT AATTGTTGCT 151 AACGTTTCAC CGTGCGTGTG TGGATATCTT CCAAGTTGTC ATTTTCTCTA 201 CCCCTCTCTT TTCCGGCGCG GTCTAACTTC CTCCAACTAG CGCATTTCCA 251 TGATAACGGG GTCCACATAG TCCGTTGTAG TGAAATTGGC ATGCCATCAG 301 GGCCAAGGCA TACATCGCGG CCGCGATAAA AATGATACGA TGATCTCCGT 351 GTGGAGCAGA CCGTGGCGAC CAGTTTGTAC CATGGAAGTT CTTCGATAGA 401 GTTATATCGA GTGCGGAATT GATGCTACGG TCACAGTGTC GTATCGTGTG 451 GTCTCCCATG TGTTTTTGAC GGTGTACCAA TTATCTTTGC ACTAATGGGC 501 TATGGAATCT TTCCTTTTAA TCAGGGGGAC ATGTTTATCC CACGCCAGTA 551 GTCTGGAATT AGAATACATA CCAACTATAT GAGCTCGAAT TCTGCATTAA 601 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 651 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 701 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 751 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 801 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 851 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 901 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 951 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 1001 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 1051 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 1101 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 1151 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 1201 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 1251 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 1301 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 1351 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 1401 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 1451 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 1501 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 1551 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 1601 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 1651 GCCTGACTCC CCGTGGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 1701 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 1751 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 1801 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC 1851 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 1901 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 1951 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 2001 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 2051 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 2101 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA 2151 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 2201 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 2251 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 2301 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 2351 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 2401 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT 2451 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 2501 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 2551 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT 2601 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG 2651 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG 2701 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA 2751 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG 2801 CATCAGAGCA GATTGTACTG AGAGTGCACC ATTCGACGCT CTCCCTTATG 2851 CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA 2901 CCGCCGCCGC AAGGAATGGT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG 2951 CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG 3001 AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC 3051 AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT 3101 AGAGGATCTG GCTAGGAATA CTGCATAGAT AACAAACGCA TCAAACACAA 3151 CTGGTGGCAC TATTTCAATC ATGTCGACAC ACCAAGACGA GAAAATTTGA 3201 GTTAAACAAG ATGCCTACAA CCCTATATTT ATTATCTTCA AGTGATCTTC 3251 TCTTCTCTTG TGCGCTGTAC GTAAATGTGT TGCAAATAAG GCATAGGGCT 3301 GAGTACAGGC ACCACACGCG AGGAAAATCA TAAGCACTAG CCGTGTCAAC 3351 TCACAGCGAC TACAGAACAA TTTTGGCCAC ACAACCCGGT GTTAGGATCT 3401 CCGAGGCCTC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC 3451 CACTATCGGC GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT 3501 CTGCGGGCGA TTTGTGTACG CCCGACAGTC CCGGCTCCGG ATCGGACGAT 3551 TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA TTGCCGTCAA 3601 CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG 3651 AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT 3701 CTGCTGCTCC ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA 3751 CCTCGTATTG GGAATCCCCG AACATCGCCT CGCTCCAGTC AATGACCGCT 3801 GTTATGCGGC CATTGTCCGT CAGGACATTG TTGGAGCCGA AATCCGCGTG 3851 CACGAGGTGC CGGACTTCGG GGCAGTCCTC GGCCCAAAGC ATCAGCTCAT 3901 CGAGAGCCTG CGCGACGGAC GCACTGACGG TGTCGTCCAT CACAGTTTGC 3951 CAGTGATACA CATGGGGATC AGCAATCGCG CATATGAAAT CACGCCATGT 4001 AGTGTATTGA CCGATTCCTT GCGGTCCGAA TGGGCCGAAC CCGCTCGTCT 4051 GGCTAAGATC GGCCGCAGCG ATCGCATCCA TGGCCTCCGC GACCGGCTGC 4101 AGAACAGCGG GCAGTTCGGT TTCAGGCAGG TCTTGCAACG TGACACCCTG 4151 TGCACGGCGG GAGATGCAAT AGGTCAGGCT CTCGCTGAAT TCCCCAATGT 4201 CAAGCACTTC CGGAATCGGG AGCGCGGCCG ATGCAAAGTG CCGATAAACA 4251 TAACGATCTT TGTAGAAACC ATCGGCGCAG CTATTTACCC GCAGGACATA 4301 TCCACGCCCT CCTACATCGA AGCTGAAAGC ACGAGATTCT TCGCCCTCCG 4351 AGAGCTGCAT CAGGTCGGAG ACGCTGTCGA ACTTTTCGAT CAGAAACTTC 4401 TCGACAGACG TCGCGGTGAG TTCAGGCTTT TTCATCACTA GACTTCGTTG 4451 CAGTTGAGTT TCTGTTTTCG GGCTTGTTGG TATACTTGTT TGATTAATGT 4501 GGCTGATAGC TAAGCTATCG CAAATATTTG TACAAGACAT GAGGGGGCAA 4551 AAAAATAAGC CAAGTGTATT TGTTAGGCTT CCGTGCCTGT TAGAGAAATA 4601 TGTAAGGAAA CGCGTGCGCT ACACAGCTTG AGCACCCATC GAATTGGGAC 4651 GAGAACATGT TAAACTCAGT ACTCACCCGA TAACCCCTAT TTTCAATGCA 4701 CGCCCACCCA GAGACACGGA ATAGCATAAA ACAACCCCAT CACTGCCACA 4751 AAATGGACAT TTTGCACAAA ACGCACTATT GACTAGTATT CGACTATACT 4801 AATAAGAGAT ACCTATCAGA CACGATATAC TGTGAAAAGG ACATTAAGCT 4851 CACTATGCAT CTGAAAACGC TTTCACCTCA CAACCTACTT ACACACACCT 4901 TTCCGCTTAA CCCGCTAGCT TTCCACCCAG CGCGGGTGCA TTCTGGCTCT 4951 TATATATACT TATTGTCATG ACAGAGTATA TTGTACTGTG TTGATAAGGG 5001 ACGGGTAACT GTATTGAAGA GCCGATGCTT TTGACATGTT AGATATAATA 5051 TGTTTTATTG TAAAGTCAAT ACAACACACA ATAGGATAAT AATGATAAAG 5101 TTAAAAAAGT ATATATAGTA ATAGAAATAT ATCTTATATA GGAAAGATTA 5151 AGCAGTAAAA GTAGCGCTTA CGGCGTACAC CCTATCAATG ATAGAGTGGT 5201 ACCCTGCACG CGCCTTCGAG TTTTTTTTCC TTTTCCCCAT TTTTTTCAAC 5251 TTGAAGACTT CAATTACACC AAAAAGTAAA ATTCACAAGC TTNNNNNNNN 5301 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5351 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5601 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5651 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5701 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5751 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5801 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5851 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5901 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 5951 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6001 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6051 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6101 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6151 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6201 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6251 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6301 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6351 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6601 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6651 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 6701 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGGA 6751 TCCTGCCCAT TTAGTTGGCT TTTCCCTTGT CTCGTGTCTT TTCCGTGGAA 6801 AGGTTCCCGG AGTAATCTGA TGGCACAGCA GGGAGGTGCG CCTGCAGGTT 6851 GGTTAGGAAG GGGGGATGAT GTAAAAGAAG AAAATGGGGG GATATCTTAT 6901 GTTTAAGAGG ATAAATAATG TGAAGGGGCT TTATATGCTT GCTTGGTTGT 6951 CTCGTTGGTG CATGGGGAAT CTGCATGTTT GCTTTGGAGC ACGCGTGGTA 7001 CATCAATGGG ATCATATCTT GCTGCCTCCC GCAGCTCACC GTGCGAGCTG 7051 CCGGGACCCC GTTTCTCATA GGCGTGTGCA CCTTCCGTCT GAGACCTGTA 7101 AATGGTTAAA AAGGAATATA TAATTACCTT TTGAAAAGTG GTAAAAAACG 7151 AATATATCTT TTTTTTTGGT TTTAATACGC TCTTTTGTGT GTATGAGAGG 7201 AATAAATAAG TGTGTGTGTG TGTGTGTGTT GTTGTTGTTG AAAAAATGAA 7251 GGACACGAAA GCGCTCATTA AGGGTGGGGA TACACCAGGC ATAATTATCC 7301 GAAAATATTT TGATGCACCA AATAAGTGAA CATACTGACG AAATCAAAGA 7351 GTTGGAGGAT GGATAGGGAG GCCTCATTGG CAGTGGTAAA TTGATTTGAC 7401 TTGAATATGA TACCCTCTTA CTGTTTCATT GCCACTACAA GTTGGTTTCC 7451 TTCCCCTGCA GGCATGCAAG CTAGGCCACA CCTATCAGCC GGTGTGTTGC 7501 CAAAGACATT CTGTATAATT TTCCAACTAC CCTAACCTGA TTCACTCATC 7551 GTTGAGCATT TGTTCTTCTA CTTTTTGACC GCAAGTGTCG TTATTAGTAT 7601 TCGGTCCTGG GCTGCAGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG 7651 GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA 7701 ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGAACTATAT CCGGATAGGG 7751 CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG 7801 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT 7851 TTTGCTGAAA GGAGAACTAT ATCCGGATTG GGCCCCTGCA G