[Under construction]

A selection of Trypanosoma brucei-specific protocols, updated on an ad hoc basis. For details of standard molecular biology protocols see, for example, Current Protocols in Molecular Biology or Short Protocols in Molecular Biology, both edited by Frederick Ausubel and published by Wiley.

Trypanosoma brucei culture & manipulation

Trypanosoma brucei growth analysis

  • Basic growth assay (& calculating a doubling time)
  • EC50 analysis (calculating a ‘half maximal effective growth-inhibitory concentration’)
  • Isobologram analysis (synergistic, additive & antagonistic inhibitor interactions)

Molecular Biology

Cell biology

Protein analysis

RNA analysis