Core Parasitology (3122) – Term 1
- ‘Human African trypanosomiasis’ – An overview of the biology of Trypanosoma brucei and the disease it causes.
Molecular Biology (3333) – Term 1
- ‘Gene Cloning’ – An introductory lecture looking at basic plasmid assembly, transfection and selection techniques.
- ‘Transfection’ – A 30 to 45 minute practical demonstration introducing transfection by electroporation, as well as the classical chemical approach; this session also covers ‘blue-white’ screening.
Recombinant DNA Techniques (3131) – Term 2
- ‘Gene Structure & Expression’ – A lecture introducing gene organisation in various organisms and some of the techniques used to explore gene organisation and expression.
Advanced Training in Molecular Biology (3158) – Term 2
Module organiser (since academic year 2013/14) and practical class leader
Lectures: chromatin structure and epigenetics, principles of gene expression control, and practical and theoretical aspects of applied molecular biology, including RNA interference, protein expression and microarrays.
Bioinformatics classes: using online analytical tools to generate hypotheses.
Laboratory practicals: Southern blotting, protein expression & western blotting.
- ‘Chromosome Structure & Organisation’ – A lecture looking at how DNA is organised and compacted in the nucleus, and how this influences gene expression.
Molecular Biology Research Applications & Progress (3160) – term 2
- ‘Trypanosoma brucei immune evasion strategies’ – The African trypanosome evades the adaptive immune system via antigenic variation of its surface coat, while the human infective sub-species employ additional strategies to circumvent the trypanolytic factors found in human serum. This lecture addresses the latest advances in these two areas.
Antimicrobial Chemotherapy (3169) – term 3
- ‘Anti-Protozoal Drug Resistance: Trypanosomatids’ – In this lecture I present our current understanding of the mechanisms of drug resistance in Leishmania and Trypanosoma, including details of some of the approaches used in this area of research. Whilst some of the drug resistance phenotypes discussed have only been seen in the laboratory, they highlight the potential vulnerabilities of the current therapeutic regimens.
- Tutorials & student presentations – ‘Developments in anti-trypanosomatid chemotherapy and drug resistance in the post-genomic era’. This is an opportunity to explore the latest developments in the field driven by the application of ‘omics technologies.
Pathogen Genomics (3460) – term 3
- ‘Functional genomics of eukaryotic pathogens’ – Using Trypanosoma brucei as an example, this lecture describes the application of high-throughput approaches to functional genomics, and how these are integrated with ‘traditional’ candidate-based low-throughput approaches.