Welcome to the Alsford lab web site…
…where you can find out about our research on the kinetoplastid parasites, Trypanosoma brucei and Leishmania. We’re particularly
interested in how these parasite interact with their host environment, especially the uptake and sub-cellular transit of host- and
parasite-derived molecules, including nutrients, drugs and innate immune factors.
Our latest work exploiting the T. brucei RNAi library to identify candidate anti-leishmanial drug efficacy determinants has been
published in the August 2019 edition of Antimicrobial Agents & Chemotherapy (after a few months on BioRxiv – our first pre-print!).
We describe 42 novel drug efficacy determinants, all of which have orthologues in Leishmania, including several proteins involved
in membrane trafficking and organisation.
Rachel’s paper describing the parasite factors that drive apolipoprotein-L1 action against Trypanosoma brucei brucei is available
at PLoS Pathogens – highlighting the importance of ubiquitination and membrane trafficking to apoL1 action.
The results of our collaborative research with Doris Rentsch’s lab (University of Bern) characterising the T. brucei amino acid
transporters, AAT10.1 and AAT2.4, and their roles in ornithine uptake and suramin and eflornithine efficacy is available at the
If you’re interested in using the bloodstream-form Trypanosoma brucei RNAi library in your research, or adapting the RIT-seq
approach to your preferred organism, extensive detailed protocols are available at Nature Protocols .
Explore our other pages…
…to find details of our cell lines & plasmids (all freely available to the research community on request), protocols and publications.
There are also extensive links to other online resources and Trypanosomatid research groups based in the UK and beyond. If your lab
is missing from this list (apologies!) or the link is out of date, drop me an email, and I’ll be sure to update the list.
Read our Blog for intermittent postings on publications, research, conferences, and anything else we might have an opinion on – all
(intelligent and informative) comments and contributions welcome!
| T: 020 7927 2699(lab) 020 7927 2352(office) | Lab333, LSHTM, Keppel Street, London WC1E 7HT.
| Department of Infection Biology | Infectious & Tropical Diseases | LSHTM