Welcome to the Alsford lab web site…
…where you can find out about our research on the African trypanosome, Trypanosoma brucei. We’re particularly interested in the
parasite’s interaction with its host environment, especially the uptake and sub-cellular transit of host- and parasite-derived molecules,
and the consequences of such encounters for the trypanosome.
The results of our collaborative research with Doris Rentsch’s lab (University of Bern) characterising the Trypanosoma brucei
amino acid transporters, AAT10.1 and AAT2.4, and their roles in ornithine uptake and suramin and eflornithine efficacy is now
available at the FASEB Journal
If you’re interested in using the bloodstream-form Trypanosoma brucei RNAi library in your research, or adapting the RIT-seq
approach to your preferred organism, extensive detailed protocols are available at Nature Protocols
Our research on the interaction between human serum trypanolytic factors and Trypanosoma brucei Inhibitor of Cysteine
Peptidase and Cathepsin-L is available at PLoS Pathogens; also discussed on LSHTM News, at the Wellcome Trust, in this
blog post, and in a commentary piece at Microbial Cell
A pRPHYG variant of our stem-loop RNAi construct is now available; this construct is compatible with any strain of Trypanosoma
brucei (bloodstream or procyclic form) expressing the Tet repressor protein
Explore our other pages…
…to find details of our cell lines & plasmids (all freely available to the research community on request), protocols and publications.
There are also extensive links to other online resources and Trypanosomatid research groups based in the UK and beyond. If your lab is
missing from this list (apologies!) or the link is out of date, drop me an email, and I’ll be sure to update the list.
Read our Blog for intermittent postings on publications, research, conferences, and anything else we might have an opinion on – all
(intelligent and informative) comments and contributions welcome!
| T: 020 7927 2699(lab) 020 7927 2352(office) | Lab333, LSHTM, Keppel Street, London WC1E 7HT.
| Pathogen Molecular Biology | Infectious & Tropical Diseases | Bloomsbury Research Institute